Value of Polyacryiamlde Gradient Gel Eiectrophoresis of Lipoproteins for Determining HDL Cholesterol, F. Callais
نویسنده
چکیده
hemolysis in patients exhibiting a concurrent acute-phase reaction as indicated by increases in C-reactive protein. The sensitivity of haptoglobin at a cutoff of 250 mgfL, however, is somewhat less than in situations where only hemolysis is present (2). Nonetheless, it is apparent that the decline in serum haptoglobin concentrations, owing to its complexing with hemoglobin and eventual removal via the reticuloendothelial system, occurs significantly faster than serum haptoglobin increases as an acute-phase reactant.
منابع مشابه
Umbilical cord blood lipoproteins. Isolation and characterization of high density lipoproteins.
High density lipoproteins (d = 1.063 to 1.21 g/ml) were isolated from both individual and pooled umbilical cord blood plasma by sequential preparative ultracentrifugation. Mean total HDL concentration was 139 ± 9.1 mg/dl, with mean values for cholesterol and triglyceride of 24.9 mg/dl and 5.0 mg/dl, respectively. The particle size distribution of HDL particles was examined by gradient gel elect...
متن کاملمقایسه ارتباط سطح سرمی مارکرهای کلسترولی و لیپیدی در بررسی اختلالات لیپوپروتئینی در بیماران بستری در بیمارستان قلب دانشگاه علوم پزشکی مازندران
Background and purpose: Considering some restrictions of serum low density lipoprotein (LDL) as a marker for Coronary Artery Disease (CAD) risk factor and also the importance of apo B as a signinificant risk factor for CAD, measurement of non-HDL cholesterol has great value as a risk factor for CAD. Non-HDL cholesterol (total cholesterol minus HDL cholesterol) contains all lipoproteins includ...
متن کاملAssociations of lipoproteins and apolipoproteins with gradient gel electrophoresis estimates of high density lipoprotein subfractions in men and women.
We examined the relations of gender and lipoproteins to subclasses of high density lipoproteins (HDLs) in a cross-sectional sample of moderately overweight men (n = 116) and women (n = 78). The absorbance of protein-stained polyacrylamide gradient gels was used as an index of mass concentrations of HDL at intervals of 0.01 nm across the entire HDL particle size range (7.2-12 nm). At least five ...
متن کاملDensity gradient characterization of the high density lipoproteins in cholesterol-fed hyper- and hyporesponding patas monkeys (Erythrocebus patas).
Diet-induced changes in high density lipoprotein (HDL) density and size were studied in patas monkeys. When the animals were switched from a moderate fat-low cholesterol diet to a high fat-high cholesterol (HFHC) diet, the plasma apoA-I levels increased initially in all of the animals. The apoA-I levels remained elevated in monkeys able to maintain their plasma cholesterol concentrations near b...
متن کاملFamilial Apolipoprotein Al
A bstract. Lipoprotein classes isolated from the plasma of two patients with apolipoprotein Al (apo Al) and apolipoprotein CIII (apo CIII) deficiency were characterized and compared with those of healthy, ageand sex-matched controls. The plasma triglyceride values for patients 1 and 2 were 31 and 51 mg/dl, respectively, and their cholesterol values were 130 and 122 mg/dl, respectively; the pati...
متن کامل